What is TMB Stab Pred?
TMB Stab Pred represents a machine learning approach developed to predict the stability of β-barrel membrane protein. This method leverages a diverse array of sequence and structure-based characteristics. By combining these features, TMB Stab Pred offers a powerful tool for predicting the stability of β-barrel membrane protein with high accuracy.
What is the dataset used in TMB Stab Pred?
A dataset comprising 11 β-barrel membrane protein has been curated from the MPTherm database, focusing on stability determinants. Firstly, the Unfolding Gibbs free energy change (ΔG0) derived from chemical denaturation data was considered, specifically in the absence of denaturant. Secondly, to ensure uniformity in physiological conditions, only data obtained within the experimental pH range of 5-9 and temperature range of 22-37°C were included. Lastly, to eliminate redundancy in protein measurements, the average (ΔG0) value was taken for instances where a protein had multiple measurements. This rigorous approach allowed for the creation of a robust and reliable dataset, setting the stage for accurate analysis and prediction of β-barrel membrane protein stability.
What is the performance of TMB Stab Pred?
In our study, we evaluated the performance of our model through a jack-knife test, resulting in a remarkable correlation coefficient of 0.96 and a mean absolute error (MAE) of 0.94 kcal/mol. These metrics attest to the model's strong predictive capabilities for stability in β-barrel membrane proteins. To validate the model's robustness and generalizability, we employed a blind test set. Encouragingly, the model demonstrated exceptional predictive accuracy on this test set, achieving an average MAE of 0.78 kcal/mol. This outcome underscores the model's reliability and potential practical utility in real-world scenarios, where accurately predicting β-barrel membrane proteins stability is of great importance.
Can TMB Stab Pred predict for alphafold structures?
Yes, the TMB Stab Pred can predict for the structures from alphafold.
Browser compatibility of TMB Stab Pred
The website is implemented in HTML, Python, PHP, JavaScript and supports recent versions of major browsers such as IE10, Firefox, Chrome and Opera.
Disclaimer
The use of this website implies you are aware of and in agreement with the disclaimer provided below: TMB Stab Pred is meant for education and research purposes only. It is a non-profit service to the scientific community, and is provided without warranty of any kind, either expressed or implied. In no event shall the authors and copyright holders be held liable for any claim, damages or any other liability arising from, out of or in connection with TMB Stab-Pred..