{"method": "X-RAY DIFFRACTION", "mutation_s_field": "No", "ec_number": "3.2.1.17", "description": "A single-domain fragment of a camelid antibody raised against wild-type human lysozyme inhibits the in vitro aggregation of its amyloidogenic variant, D67H. The structural study reveals that the binding of the antibody fragment achieves its effect by restoring the structural cooperativity characteristic of the wild-type protein. This appears to occur at least in part through the transmission of long-range conformational effects to the interface between the two structural domains of the protein. Reducing the ability of an amyloidogenic protein to form partly unfolded species can be an effective method of preventing its aggregation.", "ligstr": "", "pmid": "12917687", "remarks": "Complex of human lysozyme with camelid VHH HL6 antibody fragment", "amyloid_non_amyloid": "Non-amyloid", "type": "Inhibitor complex", "inchi": "", "resolution": "1.86", "species": "", "secondary_structure": "QVQLQESGGGSVQAGGSLRLSCSASGYTYISGWFRQAPGKEREGVAAIRSSDGTTYYADSVKGRFTISQDNAKNTVYLQMNSLKPEDTAMYYCAATEVAGWPLDIGIYDYWGQGTEVTVSS#CCCEEEECCCEEETTCCEEEEEEESSCCSEEEEEEECTTSCCEEEEEEETTTTEEEECTTTBTTEEEEEETTTTEEEEEECSCCGGGCEEEEEEEESSCSCTTCGGGCCEECCCEEEEEEC&KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV#CBCCHHHHHHHHHHTTCTTBTTBCHHHHHHHHHHHTSSBTTCEEEETTTTEEEETTTTEETTTTSBCSCCTTCCCTTCCBGGGGGSSCCHHHHHHHHHHHHSTTGGGGSHHHHHHTTTSCCGGGTTTSCC", "chain_id": "", "gene_names": "LYZ, LZM", "r_value_free": "0.23800000", "pdb_id": "1OP9", "reference": "Nature. 2003 Aug 14;424(6950):783-8", "keyword": "", "protein_name": "lysozyme ", "ligand_formula": "", "peptide_protein_sequence": "chain-ID A: QVQLQESGGGSVQAGGSLRLSCSASGYTYISGWFRQAPGKEREGVAAIRSSDGTTYYADSVKGRFTISQDNAKNTVYLQMNSLKPEDTAMYYCAATEVAGWPLDIGIYDYWGQGTEVTVSS; chain-ID B: KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV", "entry": "S-0047", "length": "121 ,  130", "uniprot_ac": "", "author": "Dumoulin, M., Last, A.M., Desmyter, A., Decanniere, K., Canet, D., Larsson, G., Spencer, A., Archer, D.B., Sasse, J., Muyldermans, S., Wyns, L., Redfield, C., Matagne, A., Robinson, C.V., Dobson, C.M.", "ligand_smiles": "", "ligand_mw": "", "alternative_name": "1, 4-beta-N-acetylmuramidase C", "ligand_name": "", "pdb_classification": "HYDROLASE", "global_stoichiometry": "Hetero 2-mer - AB ", "inchi_key": "", "ligand_id": ""}