{"method": "X-RAY DIFFRACTION", "mutation_s_field": "I56T ", "ec_number": "3.2.1.17", "description": "The crystal structure of the mutant protein was the same as the wild-type structure, except that the hydroxyl group of the introduced Thr56 formed a hydrogen bond with a water molecule in the interior of the protein. The other physicochemical properties of the mutant protein in the native state were not different from those of the wild-type protein. However, the equilibrium and kinetic stabilities of the mutant protein were remarkably decreased due to the introduction of a polar residue (Thr) in the interior of the molecule. It can be concluded that the amyloid formation of the mutant human lysozyme is due to a tendency to favor (partly or/and completely) denatured structures.", "ligstr": "", "pmid": "9010773", "remarks": "CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE I56T MUTANT", "amyloid_non_amyloid": "Amyloid", "type": "Protein", "inchi": "InChI=1S/Na/q+1", "resolution": "1.8", "species": "Homo sapiens", "secondary_structure": "KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGTFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV#CBCCHHHHHHHHHHTTCTTBTTBCHHHHHHHHHHHTSSBTTCEEEETTTTEEEETTTTEETTTTSBCSCSTTCCCTTCSBGGGGGSSSCHHHHHHHHHHTTSTTGGGGSHHHHHHTTTSCCGGGTTTSCC", "chain_id": "A", "gene_names": "LYZ, LZM", "r_value_free": "", "pdb_id": "1OUA", "reference": "J Biochem. 1996 Dec;120(6):1216-23", "keyword": "LYSOZYME", "protein_name": "Lysozyme C", "ligand_formula": "Na 1", "peptide_protein_sequence": "chain-ID A: KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGTFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV", "entry": "S-0048", "length": "130", "uniprot_ac": "P61626", "author": "Funahashi, J., Takano, K., Ogasahara, K., Yamagata, Y., Yutani, K.", "ligand_smiles": "[Na+]", "ligand_mw": "22.99", "alternative_name": "1, 4-beta-N-acetylmuramidase C", "ligand_name": "SODIUM ION", "pdb_classification": "HYDROLASE (O-GLYCOSYL)", "global_stoichiometry": "Monomer - A ", "inchi_key": "FKNQFGJONOIPTF-UHFFFAOYSA-N", "ligand_id": ""}