{"method": "X-RAY DIFFRACTION", "mutation_s_field": "T70N", "ec_number": "3.2.1.17", "description": "T70N human lysozyme is the only known naturally occurring destabilised lysozyme variant that has not been detected in amyloid deposits in human patients. The X-ray crystal structure shows that a substantial structural rearrangement results from the amino acid substitution, involving residues 45-51 and 68-75 in particular, and gives rise to a concomitant separation of these two loops of up to 6.5A. For residues 70-74, shows that the T70N substitution increases the flexibility of the peptide backbone around the site of mutation.", "ligstr": "CL:A:Cl -1:35.45:CHLORIDE ION:[Cl-]:InChI=1S/ClH/h1H/p-1:VEXZGXHMUGYJMC-UHFFFAOYSA-M", "pmid": "16126226", "remarks": "T70N human lysozyme is the only known naturally occurring destabilised lysozyme variant that has not been detected in amyloid deposits in human patients", "amyloid_non_amyloid": "Non-amyloid", "type": "Protein", "inchi": "InChI=1S/ClH/h1H/p-1", "resolution": "2.5", "species": "Homo sapiens", "secondary_structure": "KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKNPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV#CBCCHHHHHHHHHHTTCTTBTTBCHHHHHHHHHHHHSSBTTCEEEETTTTEEEETTTTEETTTSSBCSSCTTCCCTTCSBGGGGSSSSCHHHHHHHHHHTTSTTGGGGSHHHHHHTTTSCCGGGTTTTCC", "chain_id": "A", "gene_names": "LYZ, LZM", "r_value_free": "0.268", "pdb_id": "1W08", "reference": "J Mol Biol. 2005 Sep 30;352(4):823-36", "keyword": "LYSOZYME", "protein_name": "Lysozyme C", "ligand_formula": "Cl -1", "peptide_protein_sequence": "chain-ID A: KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKNPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV", "entry": "S-0069", "length": "130", "uniprot_ac": "P61626", "author": "Johnson, R., Christodoulou, J., Dumoulin, M., Caddy, G., Alcocer, M., Murtagh, G., Kumita, J.R., Larsson, G., Robinson, C.V., Archer, D.B., Luisi, B., Dobson, C.M.", "ligand_smiles": "[Cl-]", "ligand_mw": "35.45", "alternative_name": "1, 4-beta-N-acetylmuramidase C", "ligand_name": "CHLORIDE ION", "pdb_classification": "HYDROLASE", "global_stoichiometry": "Monomer - A ", "inchi_key": "VEXZGXHMUGYJMC-UHFFFAOYSA-M", "ligand_id": "CL"}