{"method": "X-RAY DIFFRACTION", "mutation_s_field": "No", "ec_number": "", "description": "For the yeast protein Sup35, conversion to amyloid-like fibrils is associated with a transmissible infection akin to that caused by mammalian prions. A seven-residue peptide segment from Sup35 forms amyloid-like fibrils and closely related microcrystals, from which the atomic structure of the cross-Beta spine is determined. It is a double Beta-sheet, with each sheet formed from parallel segments stacked in register. Side chains protruding from the two sheets form a dry, tightly self-complementing steric zipper, bonding the sheets. Within each sheet, every segment is bound to its two neighbouring segments through stacks of both backbone and side-chain hydrogen bonds. The structure illuminates the stability of amyloid fibrils, their self-seeding characteristic and their tendency to form polymorphic structures.", "ligstr": "ACY:A:C2 H4 O2:60.05:ACETIC ACID:CC(=O)O:InChI=1S/C2H4O2/c1-2(3)4/h1H3,(H,3,4):QTBSBXVTEAMEQO-UHFFFAOYSA-N;ZN:A:Zn 2:65.41:ZINC ION:[Zn+2]:InChI=1S/Zn/q+2:PTFCDOFLOPIGGS-UHFFFAOYSA-N", "pmid": "15944695", "remarks": "Structure of NNQQNY from yeast prion Sup35 with zinc acetate; Structure of the cross-beta spine", "amyloid_non_amyloid": "Amyloid", "type": "Fibril", "inchi": "InChI=1S/C2H4O2/c1-2(3)4/h1H3,(H,3,4)%%InChI=1S/Zn/q+2", "resolution": "1.3", "species": "Saccharomyces cerevisiae", "secondary_structure": "NNQQNY#CCCCCC", "chain_id": "A%%A", "gene_names": "", "r_value_free": "0.152", "pdb_id": "1YJO", "reference": "Nature. 2005 Jun 9;435(7043):773-8", "keyword": "Eukaryotic peptide chain release factor GTP-binding subunit", "protein_name": "Eukaryotic peptide chain release factor GTP-binding subunit", "ligand_formula": "C2 H4 O2%%Zn 2", "peptide_protein_sequence": "chain-ID A: NNQQNY", "entry": "S-0075", "length": "6", "uniprot_ac": "P05453", "author": "Nelson, R., Sawaya, M.R., Balbirnie, M., Madsen, A.O., Riekel, C., Grothe, R., Eisenberg, D.", "ligand_smiles": "CC(=O)O%%[Zn+2]", "ligand_mw": "60.05%%65.41", "alternative_name": "", "ligand_name": "ACETIC ACID%%ZINC ION", "pdb_classification": "PROTEIN BINDING", "global_stoichiometry": "Homo 2-mer - A2", "inchi_key": "QTBSBXVTEAMEQO-UHFFFAOYSA-N%%PTFCDOFLOPIGGS-UHFFFAOYSA-N", "ligand_id": "ACY%%ZN"}