{"method": "X-RAY DIFFRACTION", "mutation_s_field": "H119A", "ec_number": "4.6.1.18", "description": "A designed amyloid-like fibril of the well-characterized enzyme RNase A contains native-like molecules capable of enzymatic activity. In addition, these functional molecular units are formed from a core RNase A domain and a swapped complementary domain. These findings are consistent with the zipper-spine model in which a cross-Beta spine is decorated with three-dimensional domain-swapped functional units, retaining native-like structure.", "ligstr": "PO4:A:O4 P -3:94.97:PHOSPHATE ION:[O-]P(=O)([O-])[O-]:InChI=1S/H3O4P/c1-5(2,3)4/h(H3,1,2,3,4)/p-3:NBIIXXVUZAFLBC-UHFFFAOYSA-K", "pmid": "16148936", "remarks": "Crystal Structure of an Active Site Mutant of Bovine Pancreatic Ribonuclease A (H119A-RNase A) with a 10-Glutamine expansion in the C-terminal hinge-loop", "amyloid_non_amyloid": "Amyloid", "type": "Protein", "inchi": "InChI=1S/H3O4P/c1-5(2,3)4/h(H3,1,2,3,4)/p-3", "resolution": "1.8", "species": "Bos taurus", "secondary_structure": "AMAKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGGQQQQQQQQQQGNPYVPVAFDASV#CCCCCCHHHHHHHHHBCTTCSSCCSTTHHHHHHHHTTTTSSSCCSEEEEECSCHHHHHHGGGSEEECCTTSCSCEEECSSCEEEEEEEECSSCBTTBCCEEEEEEEECEEEEECCCCCCCCCCCCCCCCCCEEEEEEEC", "chain_id": "A", "gene_names": "RNASE1, RNS1", "r_value_free": "0.215", "pdb_id": "2APQ", "reference": "Nature. 2005 Sep 8;437(7056):266-9", "keyword": "Ribonuclease", "protein_name": "Ribonuclease pancreatic", "ligand_formula": "O4 P -3", "peptide_protein_sequence": "chain-ID A: AMAKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGGQQQQQQQQQQGNPYVPVAFDASV", "entry": "S-0083", "length": "139", "uniprot_ac": "P61823", "author": "Sambashivan, S., Liu, Y., Sawaya, M.R., Gingery, M., Eisenberg, D.", "ligand_smiles": "[O-]P(=O)([O-])[O-]", "ligand_mw": "94.97", "alternative_name": "RNase 1, RNase A", "ligand_name": "PHOSPHATE ION", "pdb_classification": "HYDROLASE", "global_stoichiometry": "Monomer - A ", "inchi_key": "NBIIXXVUZAFLBC-UHFFFAOYSA-K", "ligand_id": "PO4"}