{"method": "X-RAY DIFFRACTION", "mutation_s_field": "No", "ec_number": "3.2.1.17", "description": "They report results on how heat transforms globular protein, lysozyme into building block of Beta-amyloids. Light scattering experiments showed formation of lower order associated species around 50-70?\u00c2\u00b0C followed by rapid cooperativity to Beta-amyloid fibrils. crystallization drops set at higher temperatures either led to aggregates or spherulites. The latter possess an amorphous Beta-fibril rich core with thin crystalline needles projecting outwards. Diffraction of the crystalline outgrowths revealed novel dimers and trimers of lysozyme where individual chains were similar to monomer with marginal gain in Beta-sheet content. Overall this work concludes that heat induced weakly associated structures of lysozyme are the first step towards its amyloid formation. ", "ligstr": "CL:A:Cl -1:35.45:CHLORIDE ION:[Cl-]:InChI=1S/ClH/h1H/p-1:VEXZGXHMUGYJMC-UHFFFAOYSA-M;GOL:A:C3 H8 O3:92.09:GLYCEROL:C(C(CO)O)O:InChI=1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2:PEDCQBHIVMGVHV-UHFFFAOYSA-N;nan:A:Na 1:22.99:SODIUM ION:[Na+]:InChI=1S/Na/q+1:FKNQFGJONOIPTF-UHFFFAOYSA-N", "pmid": "26926993", "remarks": "Lysozyme at 318K; At 45C, in wells containing 1.2-1.3 M NaCl in mother liquid, only 1-2 spherulites were formed along with normal tetragonal and orthorhombic crystals", "amyloid_non_amyloid": "Amyloid", "type": "Protein", "inchi": "InChI=1S/ClH/h1H/p-1%%InChI=1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2%%InChI=1S/Na/q+1", "resolution": "1.71", "species": "Gallus gallus", "secondary_structure": "KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL#CBCCHHHHHHHHHHTTCTTBTTBCHHHHHHHHHHHHTTBTTCEEECTTSCEEETTTTEETTTTSBCSSCTTCCCTTCSBGGGGGSSSCHHHHHHHHHHHTSSSGGGGCHHHHHHTTTSCGGGGTTTCCC", "chain_id": "A%%A%%A", "gene_names": "LYZ", "r_value_free": "0.228", "pdb_id": "4D9Z", "reference": "Sci Rep. 2016 Mar 1;6:22475.", "keyword": "Lysozyme C", "protein_name": "Lysozyme C", "ligand_formula": "Cl -1%%C3 H8 O3%%Na 1", "peptide_protein_sequence": "chain-ID A: KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL", "entry": "S-0310", "length": "129", "uniprot_ac": "P00698", "author": "Sharma, P., Verma, N., Singh, P.K., Korpole, S., Ashish", "ligand_smiles": "[Cl-]%%C(C(CO)O)O%%[Na+]", "ligand_mw": "35.45%%92.09%%22.99", "alternative_name": "1, 4-beta-N-acetylmuramidase C, Allergen Gal d IV", "ligand_name": "CHLORIDE ION%%GLYCEROL%%SODIUM ION", "pdb_classification": "HYDROLASE", "global_stoichiometry": "Monomer - A ", "inchi_key": "VEXZGXHMUGYJMC-UHFFFAOYSA-M%%PEDCQBHIVMGVHV-UHFFFAOYSA-N%%FKNQFGJONOIPTF-UHFFFAOYSA-N", "ligand_id": "CL%%GOL%%nan"}