{"method": "SOLID-STATE NMR", "mutation_s_field": "No", "ec_number": "", "description": "The RIPK1-RIPK3 necrosome is an amyloid signaling complex that initiates TNF-induced necroptosis, serving in human immune defense, cancer, and neurodegenerative diseases. RIPK1 and RIPK3 associate through their RIP homotypic interaction motifs with consensus sequences IQIG (RIPK1) and VQVG (RIPK3).  They determined the high-resolution structure of the RIPK1-RIPK3 core. RIPK1 and RIPK3 alternately stack to form heterotypic Beta sheets. Two such Beta sheets bind together along a compact hydrophobic interface featuring an unusual ladder of alternating Ser (from RIPK1) and Cys (from RIPK3). The RIPK1-RIPK3 core is the first detailed structure of a hetero-amyloid and provides a potential explanation for the specificity of hetero- over homo-amyloid formation and a structural basis for understanding the mechanisms of signal transduction.", "ligstr": "", "pmid": "29681455", "remarks": "SSNMR Structure of the Human RIP1/RIP3 Necrosome; Human Hetero-Amyloid Signaling Complex", "amyloid_non_amyloid": "Amyloid", "type": "Fibril", "inchi": "", "resolution": "", "species": "", "secondary_structure": "PLVNIYNCSGVQVGD#CCSSEEEEEEEEEEC&TIYNSTGIQIGAYNYMEI#CEEEEEEEEEECCCCCCC&PLVNIYNCSGVQVGD#CCCCEESCEEEEEEC&TIYNSTGIQIGAYNYMEI#CEEEEEEEEEECCCCCCC&PLVNIYNCSGVQVGD#CCSCEEEEEEEEEEC&TIYNSTGIQIGAYNYMEI#CBCSEEEEEEECCCCCCC&PLVNIYNCSGVQVGD#CCSCEEEEEEEEEEC&TIYNSTGIQIGAYNYMEI#CBCSEEEEEEECCCCCCC", "chain_id": "", "gene_names": "", "r_value_free": "", "pdb_id": "5V7Z", "reference": "Cell. 2018 May 17;173(5):1244-1253.e10.", "keyword": "", "protein_name": "RIP1/RIP3 Necrosome", "ligand_formula": "", "peptide_protein_sequence": "chain-ID A: PLVNIYNCSGVQVGD; chain-ID B: TIYNSTGIQIGAYNYMEI; chain-ID C: PLVNIYNCSGVQVGD; chain-ID D: TIYNSTGIQIGAYNYMEI; chain-ID E: PLVNIYNCSGVQVGD; chain-ID F: TIYNSTGIQIGAYNYMEI; chain-ID G: PLVNIYNCSGVQVGD; chain-ID H: TIYNSTGIQIGAYNYMEI", "entry": "S-0475", "length": "15 ,  18 ,  15 ,  18 ,  15 ,  18 ,  15 ,  18", "uniprot_ac": "", "author": "Mompean, M., Li, W., Li, J., Laage, S., Siemer, A.B., Bozkurt, G., Wu, H., McDermott, A.E.", "ligand_smiles": "", "ligand_mw": "", "alternative_name": "", "ligand_name": "", "pdb_classification": "SIGNALING PROTEIN", "global_stoichiometry": "Hetero 8-mer - A4B4 ", "inchi_key": "", "ligand_id": ""}