{"method": "ELECTRON CRYSTALLOGRAPHY", "mutation_s_field": "A315T ", "ec_number": "", "description": "To uncover the structural origins of two modes of Beta-sheet-rich aggregation (reversible stress granules and irreversible pathogenic amyloid), they have determined ten structures of segments of the LCD of human TDP-43. Six of these segments form steric zippers characteristic of the spines of pathogenic amyloid fibrils; four others form LARKS, the labile amyloid-like interactions characteristic of protein hydrogels and proteins found in membraneless organelles, including stress granules. ", "ligstr": "TPO:A:C4 H10 N O6 P:199.1:PHOSPHOTHREONINE:C[C@H]([C@@H](C(=O)O)N)OP(=O)(O)O:InChI=1S/C4H10NO6P/c1-2(3(5)4(6)7)11-12(8,9)10/h2-3H,5H2,1H3,(H,6,7)(H2,8,9,10)/t2-,3+/m1/s1:USRGIUJOYOXOQJ-GBXIJSLDSA-N", "pmid": "29786080", "remarks": "Segment NFGTFS, with familial mutation A315T and phosphorylated threonine, from the low complexity domain of TDP-43, residues 312-317", "amyloid_non_amyloid": "Non-amyloid", "type": "Fibril", "inchi": "InChI=1S/C4H10NO6P/c1-2(3(5)4(6)7)11-12(8,9)10/h2-3H,5H2,1H3,(H,6,7)(H2,8,9,10)/t2-,3+/m1/s1", "resolution": "0.75", "species": "Homo sapiens", "secondary_structure": "NFGTFS#CCCCCC", "chain_id": "A", "gene_names": "", "r_value_free": "0.251", "pdb_id": "6CF4", "reference": "Nat Struct Mol Biol. 2018 Jun;25(6):463-471.", "keyword": "NFGTFS", "protein_name": "TAR DNA-binding protein 43", "ligand_formula": "C4 H10 N O6 P", "peptide_protein_sequence": "chain-ID A: NFGTFS", "entry": "S-0523", "length": "6", "uniprot_ac": "Q13148", "author": "Guenther, E.L., Cao, Q., Trinh, H., Lu, J., Sawaya, M.R., Cascio, D., Boyer, D.R., Rodriguez, J.A., Hughes, M.P., Eisenberg, D.S.", "ligand_smiles": "C[C@H]([C@@H](C(=O)O)N)OP(=O)(O)O", "ligand_mw": "199.1", "alternative_name": "", "ligand_name": "PHOSPHOTHREONINE", "pdb_classification": "PROTEIN FIBRIL", "global_stoichiometry": "Homo 10-mer - A10", "inchi_key": "USRGIUJOYOXOQJ-GBXIJSLDSA-N", "ligand_id": "TPO"}