Average Stabilizing and Destabilizing values
ΔΔG | ΔΔGH2O | ΔTm | |
---|---|---|---|
Avg Destabilizing | -1.68 | -2 | -14.89 |
Avg Stabilizing | 1.33 | 2.20 | 58.32 |
The columns represent wild-type residues. The rows represent mutated residues. For example, to find the number of A to V mutants, locate the A-row, and then the value in the V-column.
Mutation frequency count for single mutations
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0 | 17 | 19 | 50 | 29 | 236 | 22 | 26 | 45 | 77 | 24 | 16 | 84 | 14 | 17 | 106 | 52 | 98 | 7 | 15 |
C | 97 | 0 | 3 | 1 | 4 | 2 | 1 | 5 | 0 | 3 | 1 | 1 | 2 | 0 | 3 | 81 | 22 | 13 | 1 | 2 |
D | 264 | 25 | 0 | 68 | 30 | 74 | 38 | 18 | 92 | 19 | 10 | 189 | 26 | 26 | 28 | 30 | 17 | 15 | 9 | 18 |
E | 269 | 12 | 44 | 0 | 42 | 72 | 23 | 16 | 249 | 43 | 25 | 19 | 20 | 192 | 76 | 37 | 22 | 52 | 9 | 21 |
F | 231 | 7 | 7 | 13 | 0 | 7 | 11 | 19 | 9 | 85 | 13 | 9 | 2 | 4 | 3 | 22 | 7 | 26 | 67 | 52 |
G | 279 | 17 | 41 | 29 | 21 | 0 | 18 | 4 | 11 | 13 | 4 | 13 | 16 | 24 | 43 | 46 | 7 | 51 | 8 | 7 |
H | 131 | 3 | 13 | 12 | 16 | 41 | 0 | 2 | 8 | 43 | 4 | 36 | 19 | 55 | 24 | 10 | 8 | 9 | 9 | 65 |
I | 368 | 8 | 11 | 22 | 55 | 46 | 5 | 0 | 2 | 106 | 58 | 13 | 11 | 2 | 3 | 24 | 66 | 317 | 12 | 13 |
K | 277 | 7 | 22 | 128 | 60 | 84 | 39 | 39 | 0 | 21 | 53 | 50 | 15 | 113 | 58 | 27 | 26 | 31 | 26 | 11 |
L | 496 | 50 | 10 | 46 | 42 | 33 | 9 | 54 | 10 | 2 | 36 | 13 | 30 | 9 | 26 | 16 | 20 | 92 | 15 | 8 |
M | 105 | 1 | 8 | 8 | 12 | 16 | 4 | 31 | 18 | 75 | 0 | 0 | 2 | 0 | 16 | 0 | 7 | 22 | 0 | 2 |
N | 172 | 2 | 108 | 20 | 10 | 42 | 19 | 31 | 34 | 19 | 12 | 0 | 13 | 14 | 17 | 33 | 19 | 14 | 6 | 6 |
P | 212 | 6 | 5 | 3 | 5 | 62 | 5 | 2 | 0 | 22 | 0 | 1 | 0 | 1 | 9 | 56 | 11 | 13 | 2 | 2 |
Q | 109 | 3 | 7 | 29 | 10 | 44 | 12 | 6 | 22 | 48 | 4 | 16 | 15 | 0 | 16 | 6 | 2 | 8 | 1 | 5 |
R | 193 | 23 | 7 | 45 | 8 | 48 | 84 | 6 | 34 | 28 | 23 | 4 | 5 | 47 | 0 | 21 | 6 | 7 | 29 | 4 |
S | 267 | 41 | 33 | 21 | 24 | 46 | 17 | 9 | 41 | 13 | 2 | 18 | 24 | 5 | 25 | 0 | 23 | 20 | 6 | 9 |
T | 223 | 45 | 39 | 52 | 25 | 52 | 31 | 101 | 27 | 34 | 29 | 39 | 32 | 26 | 36 | 114 | 1 | 164 | 7 | 19 |
V | 535 | 40 | 31 | 42 | 54 | 111 | 16 | 155 | 28 | 112 | 79 | 28 | 14 | 16 | 27 | 66 | 149 | 6 | 15 | 33 |
W | 62 | 4 | 2 | 2 | 143 | 0 | 11 | 0 | 0 | 12 | 2 | 1 | 0 | 7 | 6 | 5 | 0 | 1 | 0 | 55 |
Y | 144 | 20 | 13 | 281 | 215 | 34 | 28 | 7 | 8 | 46 | 4 | 13 | 10 | 10 | 6 | 15 | 8 | 11 | 64 | 0 |
Substitution matrix for average stabilizing (∆∆G_H2O)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | 2.025 | 2.266 | 2.072 | 2.600 | 1.096 | 2.305 | 2.190 | 2.862 | 1.116 | 3.230 | 2.240 | 1.918 | 3.237 | 2.710 | 3.140 | 3.070 | 1.991 | 0.000 | 2.910 |
C | 2.202 | 0.000 | 0.400 | 0.000 | 0.000 | 0.000 | 0.000 | 0.360 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 1.843 | 2.330 | 0.170 | 0.000 | 0.000 |
D | 1.834 | 0.000 | 0.000 | 2.868 | 3.850 | 1.877 | 2.754 | 5.132 | 2.482 | 3.614 | 3.534 | 1.956 | 2.028 | 4.018 | 3.488 | 4.402 | 3.792 | 3.792 | 0.000 | 3.726 |
E | 1.510 | 5.543 | 2.196 | 0.000 | 3.758 | 2.099 | 2.183 | 3.483 | 2.091 | 2.924 | 3.242 | 3.232 | 1.723 | 1.476 | 3.592 | 3.224 | 3.712 | 2.757 | 0.300 | 2.593 |
F | 0.502 | 3.010 | 1.353 | 0.000 | 0.000 | 0.000 | 0.810 | 2.105 | 0.000 | 1.584 | 1.915 | 1.302 | 1.110 | 0.000 | 1.910 | 0.000 | 0.000 | 0.920 | 1.178 | 1.756 |
G | 1.725 | 4.010 | 1.857 | 1.759 | 3.488 | 0.000 | 2.882 | 3.805 | 2.854 | 3.395 | 4.020 | 2.765 | 1.455 | 2.362 | 3.472 | 1.548 | 2.855 | 2.534 | 0.000 | 3.152 |
H | 2.472 | 0.500 | 1.300 | 0.800 | 2.200 | 1.106 | 0.000 | 0.000 | 0.000 | 0.410 | 0.000 | 1.377 | 0.740 | 0.284 | 2.383 | 0.000 | 0.000 | 2.560 | 0.000 | 2.034 |
I | 1.262 | 1.405 | 2.050 | 2.990 | 3.940 | 0.470 | 2.860 | 0.000 | 3.570 | 1.266 | 3.620 | 3.300 | 0.000 | 3.510 | 3.340 | 2.680 | 3.250 | 0.903 | 0.000 | 3.840 |
K | 1.026 | 0.000 | 2.449 | 1.589 | 2.459 | 1.603 | 3.652 | 4.172 | 0.000 | 2.964 | 1.889 | 2.726 | 1.632 | 2.332 | 2.887 | 3.577 | 2.971 | 3.611 | 0.000 | 3.817 |
L | 1.463 | 2.893 | 1.393 | 1.418 | 3.452 | 1.240 | 2.280 | 3.210 | 2.000 | 0.000 | 4.130 | 1.380 | 1.560 | 1.763 | 2.517 | 1.756 | 1.918 | 1.966 | 0.810 | 2.990 |
M | 0.453 | 0.000 | 0.000 | 0.660 | 0.000 | 0.000 | 0.000 | 1.210 | 0.030 | 0.805 | 0.000 | 0.000 | 0.980 | 0.000 | 0.140 | 0.000 | 0.000 | 1.200 | 0.000 | 0.000 |
N | 1.707 | 0.000 | 1.354 | 3.145 | 4.733 | 1.676 | 4.223 | 4.098 | 4.817 | 4.783 | 4.047 | 0.000 | 1.295 | 3.772 | 4.340 | 2.704 | 3.495 | 2.978 | 0.000 | 4.557 |
P | 0.718 | 0.000 | 0.000 | 0.000 | 0.300 | 0.640 | 0.000 | 0.000 | 0.000 | 1.300 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.600 | 1.100 | 2.027 | 0.000 | 0.000 |
Q | 0.743 | 2.280 | 2.750 | 2.165 | 3.440 | 1.600 | 2.117 | 2.725 | 2.660 | 2.245 | 4.720 | 3.890 | 4.610 | 0.000 | 1.643 | 1.228 | 4.490 | 3.870 | 0.000 | 3.740 |
R | 0.300 | 0.200 | 0.000 | 0.965 | 0.000 | 1.270 | 0.060 | 0.000 | 0.200 | 0.903 | 0.000 | 0.000 | 0.000 | 1.205 | 0.000 | 0.250 | 0.000 | 0.000 | 0.600 | 0.000 |
S | 0.586 | 3.303 | 0.610 | 0.610 | 0.000 | 0.730 | 0.000 | 0.000 | 0.110 | 0.800 | 0.000 | 0.500 | 0.000 | 0.000 | 1.220 | 0.000 | 0.343 | 5.510 | 0.000 | 0.000 |
T | 2.258 | 0.600 | 2.343 | 3.894 | 3.977 | 2.900 | 3.337 | 2.841 | 3.802 | 4.418 | 4.175 | 3.718 | 1.178 | 4.020 | 3.057 | 2.739 | 0.000 | 2.351 | 0.700 | 4.038 |
V | 1.310 | 0.497 | 2.545 | 3.125 | 4.175 | 1.822 | 2.712 | 1.125 | 2.748 | 2.058 | 2.663 | 2.075 | 2.914 | 3.077 | 2.752 | 1.966 | 2.045 | 0.000 | 0.960 | 3.555 |
W | 3.100 | 0.000 | 0.170 | 0.000 | 3.056 | 0.000 | 0.000 | 0.000 | 0.000 | 0.100 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.753 |
Y | 0.985 | 0.000 | 1.138 | 0.767 | 1.024 | 1.000 | 0.860 | 2.143 | 2.810 | 1.330 | 2.223 | 0.707 | 0.000 | 1.220 | 2.257 | 6.362 | 0.817 | 1.757 | 0.150 | 0.000 |
Substitution matrix for average destabilizing (∆∆G_H2O)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | -1.148 | -0.560 | -6.338 | -1.100 | -1.544 | -0.477 | -1.458 | -1.457 | -1.022 | -0.115 | -0.330 | -3.282 | -0.337 | -0.305 | -1.175 | -2.232 | -1.929 | -0.720 | -0.545 |
C | -1.694 | 0.000 | -3.600 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -1.800 | -3.500 | 0.000 | 0.000 | 0.000 | 0.000 | -1.998 | -0.780 | -1.180 | 0.000 | -5.020 |
D | -1.583 | -2.000 | 0.000 | -2.144 | -3.025 | -1.203 | -2.200 | 0.000 | -0.928 | 0.000 | 0.000 | -2.065 | -1.600 | 0.000 | 0.000 | -1.156 | 0.000 | 0.000 | 0.000 | -4.065 |
E | -1.124 | -0.415 | -2.172 | 0.000 | -1.424 | -1.677 | -2.080 | -2.710 | -2.182 | -1.643 | -0.050 | -0.350 | -1.710 | -1.314 | -3.380 | -1.120 | -0.800 | -1.652 | -1.423 | -0.990 |
F | -2.826 | -3.110 | 0.000 | -6.360 | 0.000 | -3.263 | -1.240 | -3.225 | -1.400 | -1.996 | 0.000 | -1.080 | 0.000 | -0.460 | 0.000 | -3.065 | -3.170 | -3.107 | -1.295 | -2.260 |
G | -1.707 | -1.140 | -2.325 | -3.167 | -1.450 | 0.000 | -2.040 | 0.000 | -1.267 | -0.522 | 0.000 | -1.745 | -0.100 | -0.860 | -1.710 | -2.765 | -1.705 | -1.986 | -1.152 | -0.200 |
H | -1.021 | 0.000 | -2.200 | 0.000 | -0.710 | -2.526 | 0.000 | 0.000 | -1.677 | -2.568 | 0.000 | -1.895 | -0.600 | -0.840 | -1.000 | -1.350 | -0.220 | -2.160 | 0.000 | -1.422 |
I | -2.631 | -3.633 | -3.324 | -6.724 | -2.600 | -2.887 | 0.000 | 0.000 | -3.350 | -1.067 | -1.068 | -0.130 | 0.000 | -3.860 | -3.280 | -4.232 | -2.827 | -1.093 | -2.355 | -3.375 |
K | -0.888 | -0.533 | -1.640 | -1.625 | -0.763 | -1.161 | -1.467 | -0.805 | 0.000 | -0.030 | -2.060 | -2.758 | 0.000 | -0.761 | -0.877 | 0.000 | -1.700 | -0.912 | -0.962 | -1.050 |
L | -2.236 | -1.114 | 0.000 | -5.055 | -5.650 | -2.581 | 0.000 | -1.522 | -1.360 | 0.000 | 0.000 | -3.820 | -4.468 | -1.503 | -0.650 | -1.940 | -2.530 | -2.209 | -2.187 | 0.000 |
M | -2.290 | 0.000 | 0.000 | 0.000 | -1.600 | -3.212 | 0.000 | -2.215 | 0.000 | -1.231 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -3.730 | -0.330 | 0.000 | 0.000 |
N | -1.832 | -0.020 | -1.668 | -2.627 | -1.580 | -1.608 | -1.725 | -1.483 | -2.220 | -0.030 | -0.270 | 0.000 | -0.730 | -0.880 | -4.300 | -1.512 | -0.320 | 0.000 | -1.600 | 0.000 |
P | -1.514 | 0.000 | 0.000 | 0.000 | -0.450 | -1.172 | 0.000 | -1.700 | 0.000 | -0.667 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -1.036 | -1.250 | -1.252 | 0.000 | 0.000 |
Q | -1.095 | 0.000 | -2.670 | -0.363 | -0.700 | -1.199 | -0.800 | 0.000 | 0.000 | -1.400 | 0.000 | -1.350 | -0.765 | 0.000 | -1.145 | 0.000 | 0.000 | -4.130 | 0.000 | 0.000 |
R | -1.530 | -1.433 | 0.000 | -0.620 | -3.100 | -2.194 | -1.550 | 0.000 | -3.589 | -4.247 | -3.107 | 0.000 | -0.400 | -0.967 | 0.000 | -2.335 | -3.540 | -0.320 | -1.160 | 0.000 |
S | -1.554 | -1.075 | -1.507 | -1.207 | -1.450 | -1.066 | -1.250 | -1.410 | -0.260 | -0.685 | 0.000 | -0.810 | -0.452 | -1.460 | -0.465 | 0.000 | -0.960 | -0.917 | -0.200 | -2.270 |
T | -1.720 | -0.943 | -1.834 | -3.273 | -4.070 | -1.625 | -2.105 | -0.923 | -1.270 | -3.100 | 0.000 | -1.280 | -2.275 | -2.832 | -0.440 | -1.070 | 0.000 | -1.322 | 0.000 | 0.000 |
V | -2.012 | -2.212 | 0.000 | -6.074 | -2.688 | -2.569 | -2.055 | -0.968 | -4.800 | -0.922 | -0.957 | -1.227 | -5.000 | 0.000 | -1.460 | -3.889 | -1.786 | 0.000 | -3.463 | -1.300 |
W | -3.120 | 0.000 | 0.000 | -2.890 | -2.115 | 0.000 | -2.780 | 0.000 | 0.000 | -1.310 | -2.030 | -2.520 | 0.000 | -3.638 | -4.115 | -2.740 | 0.000 | 0.000 | 0.000 | -2.530 |
Y | -2.720 | -1.638 | -4.620 | -6.667 | -1.339 | -2.561 | -4.175 | -2.360 | -3.900 | -2.822 | -2.000 | -2.482 | -1.875 | -2.300 | -2.900 | -1.875 | -1.950 | -3.000 | -1.806 | 0.000 |
Substitution matrix for average stabilizing (∆∆G)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | 0.800 | 0.290 | 0.020 | 0.500 | 1.236 | 0.000 | 1.650 | 1.013 | 0.946 | 0.260 | 0.000 | 0.526 | 0.000 | 0.000 | 0.430 | 0.053 | 0.795 | 0.00 | 0.000 |
C | 1.690 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 1.880 | 2.327 | 0.000 | 0.00 | 0.000 |
D | 1.501 | 0.800 | 0.000 | 0.637 | 2.605 | 0.884 | 1.288 | 1.915 | 0.610 | 2.015 | 0.600 | 1.350 | 0.000 | 1.200 | 1.157 | 1.044 | 0.700 | 0.715 | 2.30 | 1.550 |
E | 0.434 | 0.910 | 0.200 | 0.000 | 1.700 | 1.550 | 0.100 | 1.650 | 0.874 | 1.257 | 1.600 | 0.000 | 0.300 | 0.494 | 2.067 | 0.203 | 1.510 | 1.037 | 1.31 | 0.600 |
F | 1.838 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.283 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.07 | 0.627 |
G | 0.476 | 0.000 | 0.000 | 0.767 | 0.000 | 0.000 | 0.470 | 0.000 | 0.000 | 0.000 | 0.000 | 0.790 | 0.000 | 0.000 | 0.240 | 1.676 | 0.000 | 0.407 | 0.00 | 0.000 |
H | 0.500 | 0.000 | 0.670 | 0.000 | 0.000 | 1.243 | 0.000 | 0.000 | 0.000 | 2.427 | 0.000 | 0.000 | 1.079 | 0.000 | 0.265 | 4.400 | 0.000 | 0.000 | 0.00 | 1.521 |
I | 3.150 | 0.000 | 0.000 | 0.000 | 0.000 | 0.170 | 0.000 | 0.000 | 0.000 | 0.725 | 0.900 | 1.945 | 0.000 | 0.000 | 0.000 | 0.000 | 1.880 | 0.208 | 0.00 | 0.000 |
K | 0.287 | 0.000 | 1.800 | 1.200 | 0.000 | 1.200 | 0.000 | 1.099 | 0.000 | 0.000 | 1.000 | 1.102 | 0.050 | 0.643 | 0.077 | 1.200 | 0.000 | 0.100 | 0.00 | 0.000 |
L | 1.787 | 0.650 | 0.000 | 0.000 | 0.390 | 0.000 | 1.390 | 0.000 | 0.000 | 0.020 | 0.050 | 0.000 | 0.690 | 0.000 | 0.445 | 0.000 | 0.000 | 0.000 | 0.00 | 0.000 |
M | 1.666 | 0.000 | 1.310 | 0.653 | 0.430 | 0.000 | 0.000 | 0.100 | 0.557 | 0.480 | 0.000 | 0.000 | 0.000 | 0.000 | 1.227 | 0.000 | 0.000 | 0.000 | 0.00 | 0.000 |
N | 0.242 | 0.000 | 0.437 | 0.400 | 1.300 | 0.290 | 0.300 | 2.956 | 0.145 | 2.560 | 2.220 | 0.000 | 0.000 | 0.000 | 0.000 | 0.485 | 0.860 | 2.055 | 0.00 | 0.000 |
P | 0.973 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 2.550 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.300 | 0.000 | 0.000 | 0.00 | 0.000 |
Q | 0.680 | 1.250 | 0.000 | 0.160 | 0.000 | 1.313 | 0.000 | 0.000 | 0.633 | 0.717 | 0.000 | 1.425 | 0.000 | 0.000 | 0.500 | 0.000 | 0.000 | 0.000 | 0.00 | 1.320 |
R | 0.435 | 0.280 | 0.000 | 0.346 | 0.000 | 0.557 | 0.313 | 0.000 | 0.115 | 0.000 | 0.100 | 0.000 | 0.000 | 0.254 | 0.000 | 0.000 | 0.000 | 0.000 | 0.00 | 0.000 |
S | 0.491 | 0.000 | 0.482 | 0.018 | 0.915 | 0.335 | 0.040 | 1.005 | 0.691 | 0.390 | 0.330 | 0.150 | 0.140 | 0.270 | 1.018 | 0.000 | 0.470 | 0.900 | 0.05 | 0.190 |
T | 0.680 | 0.780 | 0.600 | 0.208 | 3.263 | 0.000 | 0.370 | 2.432 | 0.350 | 0.450 | 0.900 | 0.207 | 0.000 | 0.380 | 0.510 | 0.440 | 1.360 | 1.176 | 1.04 | 3.340 |
V | 0.769 | 0.000 | 0.125 | 0.200 | 0.000 | 0.660 | 0.000 | 0.491 | 0.000 | 0.566 | 0.425 | 0.070 | 0.500 | 0.000 | 0.880 | 0.000 | 0.000 | 0.000 | 0.00 | 0.000 |
W | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.00 | 0.000 |
Y | 0.000 | 2.267 | 2.700 | 0.000 | 1.846 | 0.000 | 0.655 | 0.000 | 0.720 | 1.100 | 0.000 | 0.000 | 0.000 | 1.400 | 0.000 | 0.000 | 0.000 | 0.900 | 0.99 | 0.000 |
Substitution matrix for average destabilizing (∆∆G)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | -1.80 | -1.750 | -0.907 | -0.430 | -1.197 | -3.600 | 0.000 | -4.800 | -1.212 | -1.900 | -1.800 | -2.489 | -1.200 | 0.000 | -0.983 | -1.846 | -1.662 | 0.000 | -2.300 |
C | -2.775 | 0.00 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -1.432 | 0.000 | -0.910 | 0.000 | 0.000 |
D | -1.581 | 0.00 | 0.000 | -0.350 | -0.200 | -1.780 | -1.400 | -0.800 | -0.636 | -0.100 | -0.270 | -1.447 | -1.973 | -0.245 | 0.000 | -3.100 | -0.400 | -0.400 | -0.400 | 0.000 |
E | -0.526 | 0.00 | -0.200 | 0.000 | 0.000 | -5.400 | 0.000 | 0.000 | -0.606 | -1.940 | 0.000 | -0.400 | 0.000 | -0.723 | -0.600 | 0.000 | 0.000 | -2.300 | 0.000 | 0.000 |
F | -2.473 | -2.80 | 0.000 | 0.000 | 0.000 | 0.000 | -1.835 | -0.350 | 0.000 | -1.870 | -0.600 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -2.713 | -0.290 | -0.575 |
G | -1.313 | 0.00 | -1.367 | 0.000 | -1.500 | 0.000 | -0.340 | 0.000 | -1.000 | -0.210 | 0.000 | -0.960 | -2.400 | -0.287 | -3.050 | -1.690 | 0.000 | -4.660 | 0.000 | 0.000 |
H | -0.643 | 0.00 | -0.885 | -0.510 | 0.000 | -0.350 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -4.500 | -0.800 | -1.660 | -0.672 | -0.200 | -0.725 | 0.000 | -1.720 | -0.190 |
I | -2.274 | 0.00 | -2.500 | -1.622 | -1.534 | -3.443 | -1.230 | 0.000 | 0.000 | -0.910 | -1.345 | -7.630 | -2.820 | 0.000 | 0.000 | -2.360 | -3.022 | -0.819 | -1.865 | -2.927 |
K | -0.631 | 0.00 | -6.690 | -0.576 | 0.000 | -0.535 | -0.450 | -0.400 | 0.000 | 0.000 | -0.120 | 0.000 | -0.200 | -0.251 | -0.130 | -0.290 | 0.000 | 0.000 | -1.600 | 0.000 |
L | -2.445 | -2.80 | -5.367 | -0.910 | -0.489 | -5.460 | 0.000 | -1.193 | -3.900 | 0.000 | -0.918 | -2.145 | 0.000 | 0.000 | -1.727 | -3.300 | -3.325 | -1.337 | 0.000 | 0.000 |
M | -1.863 | 0.00 | 0.000 | 0.000 | 0.000 | -0.253 | 0.000 | -1.503 | -4.600 | -1.663 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -1.870 | -1.497 | 0.000 | -0.100 |
N | -2.894 | 0.00 | -0.943 | 0.000 | 0.000 | -3.440 | -0.750 | 0.000 | -0.393 | 0.000 | 0.000 | 0.000 | 0.000 | -0.080 | 0.000 | -1.285 | -0.530 | 0.000 | 0.000 | 0.000 |
P | -1.082 | 0.00 | 0.000 | 0.000 | 0.000 | -2.046 | 0.000 | 0.000 | 0.000 | -3.440 | 0.000 | -2.150 | 0.000 | 0.000 | -0.970 | -1.938 | 0.000 | -1.070 | -2.380 | -2.070 |
Q | -0.567 | 0.00 | 0.000 | -0.450 | 0.000 | -1.600 | -0.450 | 0.000 | 0.000 | -0.680 | -1.200 | -2.560 | -1.750 | 0.000 | 0.000 | -1.270 | -0.430 | -0.050 | 0.000 | -0.020 |
R | -0.858 | -2.90 | -2.900 | -1.118 | -4.200 | -1.565 | -1.952 | -2.850 | -1.624 | -1.888 | -2.090 | -3.150 | -5.750 | -0.934 | 0.000 | -5.072 | -2.950 | -2.450 | -4.350 | -4.600 |
S | -0.764 | -0.11 | -0.840 | 0.000 | -0.750 | -0.948 | -1.310 | 0.000 | 0.000 | -0.500 | 0.000 | -0.120 | -3.030 | 0.000 | 0.000 | 0.000 | -1.500 | -0.080 | 0.000 | -3.070 |
T | -1.106 | -0.90 | -0.947 | -0.800 | -2.400 | -1.832 | -2.125 | -1.767 | 0.000 | -1.600 | 0.000 | -0.908 | -2.337 | 0.000 | -1.000 | -1.011 | 0.000 | -1.643 | 0.000 | 0.000 |
V | -1.659 | -2.96 | -0.930 | -1.947 | -2.126 | -2.424 | -1.453 | -0.634 | -1.385 | -1.426 | -2.608 | -2.438 | -3.500 | 0.000 | -0.225 | -1.961 | -1.387 | 0.000 | 0.000 | -0.852 |
W | -0.400 | 0.00 | 0.000 | 0.000 | -2.483 | 0.000 | -0.500 | 0.000 | 0.000 | -4.500 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | -1.470 |
Y | -3.726 | 0.00 | 0.000 | 0.000 | -1.084 | -5.989 | -2.250 | 0.000 | 0.000 | -0.770 | 0.000 | 0.000 | -0.220 | 0.000 | 0.000 | -0.330 | 0.000 | 0.000 | -0.252 | 0.000 |
Substitution matrix for average stabilizing (∆Tm)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | 2.500 | 2.270 | 2.871 | 8.500 | 0.595 | 2.500 | 6.433 | 5.825 | 4.293 | 0.733 | 1.367 | 2.127 | 0.500 | 3.960 | 1.447 | 1.131 | 3.512 | 0.000 | 0.550 |
C | 3.006 | 0.000 | 19.800 | 0.000 | 0.000 | 1.200 | 0.000 | 0.100 | 0.000 | 0.000 | 0.000 | 13.500 | 0.000 | 0.000 | 2.600 | 2.923 | 3.200 | 0.800 | 0.000 | 0.000 |
D | 3.158 | 12.684 | 0.000 | 3.001 | 5.683 | 6.229 | 3.808 | 4.275 | 3.862 | 6.500 | 9.500 | 3.893 | 6.700 | 7.780 | 3.633 | 3.757 | 2.700 | 2.600 | 7.600 | 8.433 |
E | 1.175 | 4.650 | 1.400 | 0.000 | 2.650 | 11.800 | 3.100 | 6.850 | 7.675 | 9.683 | 4.225 | 0.000 | 1.200 | 4.358 | 11.992 | 1.908 | 5.000 | 7.805 | 5.600 | 3.300 |
F | 4.607 | 72.000 | 0.000 | 14.565 | 0.000 | 0.000 | 0.750 | 0.000 | 0.000 | 0.758 | 0.000 | 4.355 | 0.000 | 3.780 | 0.000 | 0.800 | 0.000 | 13.000 | 2.933 | 4.417 |
G | 2.165 | 2.400 | 1.386 | 1.924 | 12.700 | 0.000 | 1.767 | 0.000 | 0.000 | 15.700 | 0.000 | 2.720 | 0.000 | 2.985 | 8.654 | 3.660 | 0.500 | 7.625 | 4.700 | 15.700 |
H | 1.160 | 0.800 | 2.200 | 0.400 | 17.000 | 4.050 | 0.000 | 0.000 | 32.800 | 5.017 | 0.400 | 1.000 | 3.389 | 0.100 | 3.100 | 0.200 | 0.400 | 3.600 | 0.400 | 3.245 |
I | 0.340 | 4.200 | 0.000 | 0.000 | 2.300 | 3.800 | 1.300 | 0.000 | 0.000 | 2.255 | 8.350 | 0.000 | 2.500 | 0.000 | 0.000 | 0.000 | 40.850 | 1.601 | 0.000 | 0.000 |
K | 2.228 | 0.400 | 4.333 | 2.836 | 1.430 | 4.260 | 0.500 | 8.663 | 0.000 | 10.071 | 9.322 | 7.283 | 0.300 | 1.620 | 0.682 | 11.500 | 0.000 | 4.073 | 1.650 | 0.000 |
L | 0.700 | 23.550 | 0.000 | 0.000 | 13.562 | 0.900 | 4.600 | 1.300 | 3.750 | 0.000 | 0.000 | 3.550 | 6.900 | 0.000 | 2.200 | 4.300 | 0.000 | 0.000 | 11.000 | 5.000 |
M | 2.533 | 0.000 | 3.043 | 1.432 | 0.867 | 0.000 | 4.000 | 1.075 | 1.510 | 1.210 | 0.000 | 0.000 | 2.900 | 0.000 | 10.400 | 0.000 | 6.790 | 5.050 | 0.000 | 0.000 |
N | 1.333 | 0.000 | 1.426 | 1.267 | 3.300 | 3.300 | 1.100 | 12.167 | 2.967 | 6.650 | 7.692 | 0.000 | 2.900 | 0.820 | 1.397 | 2.043 | 3.758 | 8.850 | 4.960 | 0.000 |
P | 2.133 | 3.900 | 0.000 | 1.300 | 0.000 | 3.350 | 0.000 | 0.000 | 0.000 | 4.920 | 0.000 | 0.000 | 0.000 | 0.000 | 2.300 | 0.367 | 0.533 | 0.000 | 0.000 | 0.000 |
Q | 1.600 | 4.700 | 0.800 | 3.400 | 0.000 | 5.033 | 1.700 | 2.300 | 4.960 | 3.954 | 0.000 | 6.120 | 0.000 | 0.000 | 3.438 | 6.000 | 0.000 | 4.100 | 0.000 | 5.900 |
R | 0.800 | 1.000 | 1.600 | 1.350 | 0.200 | 1.833 | 2.435 | 3.200 | 1.100 | 0.250 | 0.300 | 0.000 | 0.000 | 7.000 | 0.000 | 0.500 | 0.000 | 1.210 | 9.240 | 0.000 |
S | 2.022 | 1.609 | 4.153 | 0.100 | 2.572 | 2.700 | 7.810 | 4.652 | 12.379 | 1.090 | 0.920 | 0.500 | 1.662 | 0.750 | 9.227 | 0.000 | 1.872 | 7.898 | 2.025 | 1.020 |
T | 1.530 | 5.328 | 5.450 | 0.815 | 3.532 | 21.100 | 3.910 | 3.732 | 5.529 | 3.984 | 3.802 | 4.432 | 4.320 | 3.850 | 9.112 | 1.746 | 0.000 | 2.167 | 6.340 | 10.597 |
V | 1.435 | 6.700 | 0.360 | 0.520 | 0.800 | 1.600 | 0.000 | 2.257 | 1.250 | 4.491 | 2.140 | 0.200 | 3.700 | 0.000 | 2.900 | 0.650 | 0.975 | 0.000 | 6.100 | 0.000 |
W | 1.600 | 0.000 | 2.200 | 0.000 | 2.733 | 0.000 | 0.200 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 10.000 |
Y | 1.950 | 6.840 | 14.500 | 17.480 | 5.958 | 0.000 | 2.980 | 0.000 | 6.100 | 3.850 | 0.000 | 0.000 | 0.000 | 14.200 | 0.000 | 1.600 | 2.000 | 5.000 | 3.459 | 0.000 |
Substitution matrix for average destabilizing (∆Tm)
A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 0.000 | -4.383 | -7.367 | -4.838 | -12.567 | -3.618 | -3.450 | -10.633 | -9.625 | -8.746 | -5.540 | -2.967 | -7.981 | -3.833 | -1.400 | -7.037 | -8.690 | -8.073 | -11.100 | -3.775 |
C | -2.459 | 0.000 | 0.000 | 0.000 | -4.900 | -4.800 | -11.500 | -5.000 | 0.000 | -2.000 | 0.000 | 0.000 | -4.850 | 0.000 | -11.400 | -7.391 | 0.000 | -4.900 | 0.000 | 0.000 |
D | -5.396 | 0.000 | 0.000 | -1.871 | -2.486 | -6.953 | -8.100 | -3.450 | -1.550 | -1.900 | -1.570 | -4.211 | -7.778 | -4.620 | -2.967 | -5.483 | -1.300 | -5.820 | -3.400 | -6.667 |
E | -2.768 | -0.900 | -4.140 | 0.000 | -4.800 | -6.890 | -17.033 | -9.533 | -4.002 | -10.633 | -0.500 | -3.250 | -13.550 | -4.727 | -6.717 | -12.300 | -2.233 | -4.550 | 0.000 | -7.133 |
F | -9.348 | -13.000 | 0.000 | 0.000 | 0.000 | 0.000 | -18.080 | -5.900 | 0.000 | -8.655 | -4.320 | -3.200 | 0.000 | 0.000 | -1.200 | -22.814 | -11.100 | -8.389 | -5.962 | -5.367 |
G | -4.872 | -4.300 | -4.988 | -10.000 | -7.200 | 0.000 | -3.050 | 0.000 | -4.500 | -5.200 | 0.000 | 0.000 | -10.133 | -2.760 | -7.155 | -4.836 | 0.000 | -9.883 | -2.800 | -5.500 |
H | -2.220 | -0.200 | -3.400 | -2.550 | -2.367 | -9.981 | 0.000 | -3.400 | -1.025 | -7.927 | -3.567 | -8.700 | -6.500 | -2.110 | -2.490 | -0.423 | -2.380 | -4.833 | -7.250 | -5.686 |
I | -6.686 | -0.200 | -7.500 | -4.766 | -10.670 | -10.592 | -4.400 | 0.000 | 0.000 | -9.509 | -3.853 | -11.950 | -9.200 | 0.000 | 0.000 | -14.533 | -8.606 | -4.675 | -8.500 | -9.733 |
K | -3.030 | -2.767 | 0.000 | -5.086 | -2.400 | -8.789 | -1.750 | -2.625 | 0.000 | 0.000 | -3.800 | -3.646 | -4.880 | -2.440 | -0.883 | -2.500 | -2.414 | -1.475 | -7.736 | 0.000 |
L | -7.609 | -9.010 | -16.700 | -5.700 | -5.033 | -16.760 | -5.040 | -3.255 | -10.567 | 0.000 | -2.462 | -9.800 | -12.525 | -14.800 | -5.116 | -17.900 | -13.150 | -5.682 | -15.300 | -2.800 |
M | -5.366 | 0.000 | 0.000 | 0.000 | -5.160 | -5.946 | 0.000 | -3.526 | -13.950 | -4.414 | 0.000 | 0.000 | 0.000 | 0.000 | -11.800 | 0.000 | -13.000 | -6.106 | 0.000 | -0.100 |
N | -5.461 | -3.600 | -3.326 | -2.067 | -6.833 | -13.554 | -1.700 | -1.160 | -7.833 | -8.680 | 0.000 | 0.000 | -3.200 | -1.933 | -9.300 | -6.971 | -3.400 | -8.467 | -6.600 | -4.020 |
P | -5.129 | -2.000 | -1.300 | -0.600 | -0.550 | -5.188 | -8.640 | -2.000 | 0.000 | -5.480 | 0.000 | 0.000 | 0.000 | -0.600 | -3.433 | -3.871 | -2.650 | -8.100 | -6.500 | -6.800 |
Q | -2.283 | 0.000 | 0.000 | -1.383 | -1.350 | -5.675 | -3.733 | -0.500 | -1.700 | -1.900 | -2.700 | -13.467 | -4.617 | 0.000 | -1.100 | 0.000 | 0.000 | -11.067 | 0.000 | -0.100 |
R | -4.071 | -5.385 | -4.525 | -6.838 | -12.600 | -6.135 | -8.588 | -4.667 | -1.744 | -3.833 | -3.828 | -8.000 | -16.050 | -4.156 | 0.000 | -4.518 | -7.400 | -5.600 | -9.275 | -12.750 |
S | -3.299 | -6.205 | -5.684 | -1.150 | -6.297 | -6.956 | -4.900 | -6.800 | -1.033 | -7.300 | 0.000 | -1.100 | -7.564 | -2.000 | -7.325 | 0.000 | -5.180 | -4.240 | -3.000 | -10.767 |
T | -3.327 | -3.084 | -4.638 | -2.600 | -5.733 | -10.151 | -4.617 | -4.996 | -12.600 | -4.567 | -5.325 | -5.488 | -7.950 | -5.550 | -6.150 | -3.588 | 0.000 | -4.260 | -1.600 | 0.000 |
V | -6.961 | -6.800 | -2.633 | -8.125 | -7.825 | -15.018 | -6.875 | -2.407 | -19.333 | -3.346 | -5.950 | -2.067 | -22.600 | 0.000 | -1.287 | -10.076 | -7.394 | -9.033 | -6.620 | -3.167 |
W | -10.454 | -8.200 | 0.000 | 0.000 | -7.582 | 0.000 | -6.667 | 0.000 | 0.000 | -6.400 | 0.000 | 0.000 | 0.000 | 0.000 | -8.223 | -16.033 | 0.000 | -8.300 | 0.000 | -7.462 |
Y | -8.437 | -2.850 | -10.300 | -16.100 | -3.606 | -17.329 | -11.600 | -7.700 | -13.900 | -6.782 | 0.000 | -7.900 | -1.100 | -11.300 | -10.200 | -4.945 | 0.000 | 0.000 | -4.200 | 0.000 |