What is TMH Stab Pred?
TMH Stab Pred represents a machine learning approach developed to predict the stability of α-helical membrane proteins. This method leverages a diverse array of sequence and structure-based characteristics. By combining these features, TMH Stab Pred offers a powerful tool for predicting the stability of α-helical membrane proteins with high accuracy.
What is the dataset used in TMH Stab Pred?
A dataset comprising 38 α-helical membrane proteins has been curated from the MPTherm database, focusing on stability determinants. Firstly, the Unfolding Gibbs free energy change (ΔG0) derived from chemical denaturation data was considered, specifically in the absence of denaturant. Secondly, to ensure uniformity in physiological conditions, only data obtained within the experimental pH range of 5-9 and temperature range of 22-37°C were included. Lastly, to eliminate redundancy in protein measurements, the average (ΔG0) value was taken for instances where a protein had multiple measurements. This rigorous approach allowed for the creation of a robust and reliable dataset, setting the stage for accurate analysis and prediction of α-helical membrane protein stability.
What is the performance of TMH Stab Pred?
In our study, we evaluated the performance of our model through a jack-knife test, resulting in a remarkable correlation coefficient of 0.89 and a mean absolute error (MAE) of 1.21 kcal/mol. These metrics attest to the model's strong predictive capabilities for stability in α-helical membrane proteins. To validate the model's robustness and generalizability, we employed a blind test set. Encouragingly, the model demonstrated exceptional predictive accuracy on this test set, achieving an average MAE of 0.51 kcal/mol. This outcome underscores the model's reliability and potential practical utility in real-world scenarios, where accurately predicting α-helical membrane protein stability is of great importance.
Can TMH Stab Pred predict for alphafold structures?
Yes, the TMH Stab Pred can predict for the structures from alpha fold.
Browser compatibility of TMH Stab Pred
The website is implemented in HTML, Python, PHP, JavaScript and supports recent versions of major browsers such as IE10, Firefox, Chrome and Opera.
Disclaimer
The use of this website implies you are aware of and in agreement with the disclaimer provided below: TMH Stab Pred is meant for education and research purposes only. It is a non-profit service to the scientific community, and is provided without warranty of any kind, either expressed or implied. In no event shall the authors and copyright holders be held liable for any claim, damages or any other liability arising from, out of or in connection with TMH Stab-Pred..