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Job ID:

a unique name to identify this job

Input protein sequence:

Input PDB id:

Example: 1BA4 (Aβ40)

Select a PDB file:

Example: download Aβ40

Chain Name

("A" for sequence predictions)

Select the Threshold for the ANuPP predictions:

0.45

is it a variable light chain sequence of antibody? (includes V_LAmY-Pred predictions)

General information required for kinetics prediction

DO you have PSSM file?

Example: download Aβ40 PSSM file

PSSM file is optional. However, it can greatly improve the aggregation kinetics prediction

Experimental conditions for aggregation kinetics:

Temperature

Ionic concentration (in mM)

Protein concentration (in μM)

Note: Experimental conditions will be used for the prediction of absolute aggregation rate and classification of aggregation rate enhancer/mitigator mutations (for calculating a feature from Tango software)

Prediction of absolute aggregation rate

Predict absolute aggregation rate for the above sequence/structure?

Effect of point mutation on aggregation kinetics

Do you want to analyze the effect of mutation on aggregation rate?

Point mutation list (Example format is "A2T;Q15A")

mutate all to gatekeeper residues

Foldx updated pending on: Jan 2027

a unique name to identify this job

Prediction of aggregation-prone regions and aggregation propensity

Load example sequence

Example: 1BA4 (Aβ40)

Example: download Aβ40

("A" for sequence predictions)

clear PDB file

Prediction of aggregation kinetics

General information required for kinetics prediction

Example: download Aβ40 PSSM file

clear PSSM file

Prediction of absolute aggregation rate

Effect of point mutation on aggregation kinetics

Load example mutation for Aβ40